Biochemistry and Structural Biology  Back to Main

Faculty Detail    
Campus Address BBRB 416 Zip 2170
Phone  (205) 975-5327
Other websites Lab

Faculty Appointment(s)
Appointment Type Department Division Rank
Center  Comprehensive Cancer Center  Comprehensive Cancer Center Professor
Primary  Microbiology  Microbiology Professor

Graduate Biomedical Sciences Affiliations
Biochemistry and Molecular Genetics Program 
Biochemistry and Structural Biology 
Cell, Molecular, & Developmental Biology 
Cellular and Molecular Biology Program 
Hughes Med-Grad Fellowship Program 
Medical Scientist Training Program 

Biographical Sketch 
Peter E. Prevelige (b. 1956), Professor of Microbiology, completed undergraduate studies in biology at Lehigh University. His graduate studies were done at Brandeis University (Ph.D., Biochemistry, 1985) on histone structure with Dr. G.D. Fasman. His postdoctoral work, on virus capsid assembly, was done at MIT under the direction of Dr. J. King. He held the position of Staff Scientist at Boston Biomedical Research Institute from 1992-1995 and joined the UAB faculty in 1995.

Society Memberships
Organization Name Position Held Org Link
Materials Research Society     
Protein Society     
Biophysical Society     

Research/Clinical Interest
Structural Biology of Viral Assembly and Infection
The assembly of protein subunits into polymeric supramolecular structures is controlled both temporally and positionally. Control is achieved through the use of highly specific protein/protein recognition events, and well-defined assembly pathways. The manner in which this information is encoded in the protein subunits is not well understood, despite the fact that interfering with these pathways holds promise therapeutically for indications as diverse as viral & bacterial infections, alzheimers disease, and neoplasia. We take an interdisciplinary approach employing an array of biochemical, biophysical, genetic, and structural tools to study these processes. Two viral systems with distinctive assembly pathways are under active investigation. One system, the Salmonella bacteriophage P22 which assembles into well defined non-enveloped stable icosahedral capsids typifies tightly controlled assembly pathways while the other, HIV which forms fragile polymorphous enveloped capsids suggests more flexibility in assembly. Comparison of these systems will illuminate different biological strategies for control of capsid assembly, and will ultimately lead to novel antiviral approaches which directly target capsid assembly.

Selected Publications 
Publication PUBMEDID
Directed self-assembly of CdS quantum dots on bacteriophage P22 coat protein templates. Kale A, Bao Y, Zhou Z, Prevelige PE, Gupta A. Nanotechnology. 2013 Feb 1;24(4):045603. doi: 10.1088/0957-4484/24/4/045603. Epub 2013 Jan 7.  23296127 
Coconfinement of Fluorescent Proteins: Spatially Enforced Communication of GFP and mCherry Encapsulated within the P22 Capsid.O'Neil A, Prevelige PE, Basu G, Douglas T.Biomacromolecules. 2012 Dec 10;13(12):3902-7. doi: 10.1021/bm301347x. Epub 2012 Nov 9.  23121071  
Use of the interior cavity of the P22 capsid for site-specific initiation of atom-transfer radical polymerization with high-density cargo loading. Lucon J, Qazi S, Uchida M, Bedwell GJ, LaFrance B, Prevelige PE Jr, Douglas T.Nat Chem. 2012 Oct;4(10):781-8. doi: 10.1038/nchem.1442. Epub 2012 Aug 26.  23000990 
P22 viral capsids as nanocomposite high-relaxivity MRI contrast agents. Qazi S, Liepold LO, Abedin MJ, Johnson B, Prevelige P, Frank JA, Douglas T.Mol Pharm. 2013 Jan 7;10(1):11-7. doi: 10.1021/mp300208g. Epub 2012 Jul 16  22656692 
Nanoreactors by programmed enzyme encapsulation inside the capsid of the bacteriophage P22. Patterson DP, Prevelige PE, Douglas T. ACS Nano. 2012 Jun 26;6(6):5000-9. doi: 10.1021/nn300545z. Epub 2012 May 31.  22624576 
A retroviral chimeric capsid protein reveals the role of the N-terminal β-hairpin in mature core assembly. Cortines JR, Monroe EB, Kang S, Prevelige PE Jr. J Mol Biol. 2011 Jul 22;410(4):641-52. doi: 10.1016/j.jmb.2011.03.052.  21762805 
Three-dimensional structure of a viral genome-delivery portal vertex. Olia AS, Prevelige PE Jr, Johnson JE, Cingolani G. Nat Struct Mol Biol. 2011 May;18(5):597-603. doi: 10.1038/nsmb.2023. Epub 2011 Apr 17.  21499245 
Hydrogen/deuterium exchange analysis of HIV-1 capsid assembly and maturation. Monroe EB, Kang S, Kyere SK, Li R, Prevelige PE Jr.Structure. 2010 Nov 10;18(11):1483-91. doi: 10.1016/j.str.2010.08.016.  21070947 
Myristate exposure in the human immunodeficiency virus type 1 matrix protein is modulated by pH. Fledderman EL, Fujii K, Ghanam RH, Waki K, Prevelige PE, Freed EO, Saad JS. Biochemistry. 2010 Nov 9;49(44):9551-62. doi: 10.1021/bi101245j.  20886905 
Peering down the barrel of a bacteriophage portal: the genome packaging and release valve in p22. Tang J, Lander GC, Olia AS, Li R, Casjens S, Prevelige P Jr, Cingolani G, Baker TS, Johnson JE. Structure. 2011 Apr 13;19(4):496-502. doi: 10.1016/j.str.2011.02.01  21439834