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Faculty Detail    
Name RALPH PATTERSON BUCY
Professor		  
Campus Address WP P230
Phone  (205) 975-6236
E-mail  bucy@uab.edu
Other websites
     

Education
Undergraduate  Austin College    1975  B.A. 
Graduate  Washington University School of Medicine    1981  M.D., PhD. 
Residency  Barnes Hospital    1984  Pathology Residency 

Certifications
American Board of Pathology, Anatomic Pathology  1987 


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Clinical Pathology  Laboratory Medicine Professor Emeritus

Graduate Biomedical Sciences Affiliations
Cellular and Molecular Biology Program 
Hughes Med-Grad Fellowship Program 
Immunology 
Integrative Biomedical Sciences 
Molecular and Cellular Pathology Program 

Biographical Sketch 
R. Pat Bucy (b. 1953) is a Professor in the Department of Pathology, with secondary appointments in Microbiology and Medicine. He received the B.S. degree in Chemistry from Austin College (Sherman, TX) in 1975 and the M.D. and Ph.D. degrees from Washington University (St. Louis, MO) in 1981. His doctoral work was on the mechanism of Ir gene control of the immune response to insulin. He completed a residency in Anatomic Pathology at Barnes Hospital in St. Louis. He stayed in St. Louis as a Faculty member at Washington University and worked with P. E. Lacy on transplantation of the islets of Langerhans. He came to UAB in 1987. He is the Director of the UAB Hospital Flow Cytometry Lab.

Society Memberships
Organization Name Position Held Org Link
American Association of Immunologists  Member   
American Society for Investigative Pathology  Member   
 



Research/Clinical Interest
Title
T cell Regulation of Immune Responses
Description
I am interested in the regulation of immune responses by T cells, particularly the forms of regulation that develop in vivo in situations with chronic antigen presence. Conventional experimental systems have used model antigens given in discreet inoculations so that the clearance of antigen is the dominant overall control mechanism. In physiological situations such as solid organ transplantation, chronic viral diseases, and organ specific autoimmune diseases, antigen is usually not cleared, but the immune system develops various control mechanisms that limit immune damage. A major model system is analysis of the regulatory mechanisms operative in solid organ allograft rejection, using vascularized murine heart transplants. Our lab has produced multiple novel strains of mice including TCR Transgenic lines, mice that express the relevant transplant antigen recognized by the TCR Tg T cells as a transgene, allelic marker systems to track T cells and antigen presenting cells in vivo, selected mice with knockouts of several relevant control molecules. Current studies focus on conditions of stimulation which give rise to regulatory functions in these TCR Tg T cells and how such these induced Treg cells mediate supporession of other immune responses. In these studies, multiple techniques are used including extensive use of flow cytometry, multiple label immunofluorescence in tissue sections, in situ hybridization analysis of viral and cellular RNA species, quantitative-competitive RT-PCR, and cell culture techniques.

Selected Publications 
Publication PUBMEDID
Chai, J. G., Ratnasothy, K., Bucy, R. P., Noelle, R. J., Lechler, R., and Lombardi, G. Allospecific CD4(+) T cells retain effector function and are actively regulated by Treg cells in the context of transplantation tolerance. Eur.J.Immunol. 45: 2017-2027 2015.   25944401 
Honjo K, Kubagawa Y, Suzuki Y, Takagi M, Ohno H, Bucy RP, Izui S, Kubagawa H. Enhanced autoantibody production and Mott cell formation in FcMuR-deficient autoimmune mice. Int Immunol. 26:659-672. 2014.   24994818 
Hattori, Y., Bucy, R. P., Kubota, Y., Baldwin Iii, W. M., and Fairchild, R. L. Antibody-Mediated Rejection of Single Class I MHC-Disparate Cardiac Allografts. Am.J.Transplant. 12(8), 2017-2028. 2012.  22578247 
Ovalle, F., Jr., Patel, A., Pollins, A., de la Torre, J., Vasconez, L., Hunt, T. R., III, Bucy, R. P., Shack, R. B., and Thayer, W. P. A simple technique for augmentation of axonal ingrowth into chondroitinase-treated acellular nerve grafts using nerve growth factor. Ann.Plast.Surg. 68(5), 518-524. 2012.  22531407 
Tsang, J.Y.S., Ratnasothy, K., Li, D., Chen, Y., Bucy, R.P., Lau, K.F., Smyth, L., Lombardi, G., Lechler, R., Tam, P.K.H. The Potency of Allospecific Tregs Cells Appears to Correlate with T cell Receptor Functional Avidity. Am. J. Transplantation. 11:1610-1620. (2011).   21797973 
Macatangay BJ, Zheng L, Rinaldo CR, Landay AL, Pollard RB, Pahwa S, Lederman MM, Bucy RP. A Comparison of Immunologic Assays for Detecting Immune Responses in HIV Immunotherapeutic Studies: ACTG Trial A5181. Clin Vaccine Immunol. 17:1452-1459. (2010).   20631337 
Twigg, H. L., Schnizlein-Bick, C. T., Weiden, M., Valentine, F., Wheat, J., Day, R. B., Rominger, H., Zheng, L., Collman, R. G., Coombs, R. W., Bucy, R. P., Rezk, N. L., and Kashuba, A. D. Measurement of antiretroviral drugs in the lungs of HIV-infected patients. HIV.Ther. 4, 247-251. (2010).   20436781 
Tanriver,Y.; Ratnasothy,K.; Bucy,R.P.; Lombardi,G.; Lechler,R. Targeting MHC class I monomers to dendritic cells inhibits the indirect pathway of allorecognition and the production of IgG alloantibodies leading to long-term allograft survival. J. Immunol. 184:1757-1764 (2010).   20083658 
Gandhi, R. T., O'Neill, D., Bosch, R. J., Chan, E. S., Bucy, R. P., Shopis, J., Baglyos, L., Adams, E., Fox, L., Purdue, L., Marshak, A., Flynn, T., Masih, R., Schock, B., Mildvan, D., Schlesinger, S. J., Marovich, M. A., Bhardwaj, N., and Jacobson, J. M. A randomized therapeutic vaccine trial of canarypox-HIV-pulsed dendritic cells vs. canarypox-HIV alone in HIV-1-infected patients on antiretroviral therapy. Vaccine (2009).  19450647 
Brennan TV, Jaigirdar A, Hoang V, Hayden T, Liu FC, Zaid H, Chang CK, Bucy RP, Tang Q, Kang SM. Preferential priming of alloreactive T cells with indirect reactivity. Am. J. Transplantation. 9:709-718 (2009)   19344462 
Tsang, J. Y., Tanriver, Y., Jiang, S., Xue, S. A., Ratnasothy, K., Chen, D., Stauss, H. J., Bucy, R. P., Lombardi, G., and Lechler, R. Conferring indirect allospecificity on CD4+CD25+ Tregs by TCR gene transfer favors transplantation tolerance in mice. J Clin.Invest. 118:3619-3628 (2008).   18846251 
Kapp, J. A. and Bucy, R. P. CD8+ suppressor T cells resurrected. Hum.Immunol. 69:715-720 (2008).  18817830 
Twigg, H. L., Weiden, M., Valentine, F., Schnizlein-Bick, C. T., Bassett, R., Zheng, L., Wheat, J., Day, R. B., Rominger, H., Collman, R. G., Fox, L., Brizz, B., Dragavon, J., Coombs, R. W., and Bucy, R. P. Effect of Highly Active Antiretroviral Therapy on Viral Burden in the Lungs of HIV-Infected Subjects. J.Infect.Dis. 197:109-116 (2008).  18171293 
Kapp, J. A., Honjo, K., Kapp, L. M., Goldsmith, K., and Bucy, R. P. Antigen, in the presence of TGFb; induces up-regulation of FoxP3gfp+ in CD4+ TCR transgenic T cells that mediate linked-suppression of CD8+ T cell responses. J.Immunol. 179:2105-2114 (2007).   17675469 
Mitsuyasu, R., Gelman, R., Cherng, D. W., Landay, A., Fahey, J., Reichman, R., Erice, A., Bucy, R. P., Kilby, J. M., Lederman, M. M., Hamilton, C. D., Lertora, J., White, B. L., Tebas, P., Duliege, A. M., and Pollard, R. B. The virologic, immunologic, and clinical effects of interleukin 2 with potent antiretroviral therapy in patients with moderately advanced human immunodeficiency virus infection: a randomized controlled clinical trial--AIDS Clinical Trials Group 328. Arch.Intern.Med. 167:597-605 (2007).   17389292 
Kapp, J. A., Honjo, K., Kapp, L. M., Xu, X. Y., Cozier, A., and Bucy, R. P. TCR transgenic CD8+ T cells activated in the presence of TGFb express FoxP3 and mediate linked suppression of primary immune responses and cardiac allograft rejection. Int.Immunol. 18:1549-1562 (2006).  16966495 
J. M. Jacobson, R. P. Bucy, J. Spritzler, M. S. Saag, J. J. Eron, R.W. Coombs, R. Wang L. Fox, S.Cu-Uvin, S. E. Cohn, D. Mildvan, D. O’Neill, J. Janik, D.K. O’Connor, C. Di Vita, I. Frank. Evidence that Intermittent Structured Treatment Interruption (STI), But Not Immunization with ALVAC-HIV vCP1452, Promotes Host Control of HIV Replication: The Results of ACTG 5068 J. Infect. Dis. 194:623-632 (2006)  16897661 
John T. Bates and R. P. Bucy Enhanced responsiveness to antigen contributes more to immunological memory in CD4 T cells than increases in the number of cells. Immunology 116:318-327 (2005)  16236121 
Bucy, R. P. Viral and cellular dynamics in HIV disease. Curr.HIV./AIDS Rep. 1:40-46 (2004).  16091222 
Honjo, K., Xu, X. Y., Kapp, J. A., and Bucy, R. P. Activation and migration of allo-peptide specific TCR transgenic T cells in cardiac allograft rejection. Cell Immunol. 230:44-55 (2004).  15541718 
Honjo, K., Xu, X. Y., Kapp, J. A., and Bucy, R. P. Evidence for cooperativity in the rejection of cardiac grafts mediated by CD4 TCR Tg T cells specific for a defined allopeptide. Am.J Transplant. 4:1762-1768 (2004).   15476474 
Honjo, K., Xu, X. Y., and Bucy, R. P. CD4+ TCR transgenic T cells alone can reject vascularized heart transplants via the indirect pathway of alloantigen recognition. Transplantation 77:452-455 (2004).   14966425