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Faculty Detail    
Name JAMES A. MOBLEY
Professor, Department of Anesthesiology and Perioperative Medicine
Division of Molecular and Translational Biomedicine
Director, UAB O'Neal CCC MS/ Proteomics Shared Facility
Director, UAB Heersink SOM IRCP MS/ Proteomics Shared Facility		  
Campus Address THT 521 Zip 0006
Phone
E-mail  mobleyja@uab.edu
Other websites PROT Shared Facility
     

Education
Undergraduate  Oregon State University    1991  ACS Chemistry. B.S. 
Graduate  The Ohio State University    2000  Medicinal Chemistry & Pharmacognosy. Ph.D. 


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Anesthesiology Chair Office  Anesthesiology Chair Office Professor
Secondary  Chemistry  Chemistry Assistant Professor
Secondary  Med - Preventive Medicine  Med - Preventive Medicine Assistant Professor
Secondary  Pharmacology/Toxicology Chair's Office  Pharmacology/Toxicology Chair's Office Assistant Professor
Center  Comp Arthritis, MSK, Bone & Autoimmunity Ctr  Comp Arthritis, MSK, Bone & Autoimmunity Ctr Professor
Center  Comprehensive Cancer Center  Comprehensive Cancer Center Professor
Center  Med - Cardiovascular Disease  Ctr Cardiovasc Bio (Org Ret) Professor
Center  Ctr for Clinical & Translational Sci  Ctr for Clinical & Translational Sci Professor
Center  Nephrology Research & Training Center  Nephrology Research & Training Center Professor

Graduate Biomedical Sciences Affiliations
Biochemistry and Structural Biology 

Biographical Sketch 
Dr. Mobley obtained his B.S. in Chemistry at Oregon State University. He obtained a Ph.D. in Medicinal Chemistry and Pharmacology from The Ohio State University with an emphasis on hormonal induced carcinogesis. Prior to joining the faculty at UAB in 2006, Dr. Mobley worked as a faculty member at UMass Medical School and also at Vanderbilt. His early and continued focus has been on biomarker and small molecule discovery in cancer research.

Society Memberships
Organization Name Position Held Org Link
American Association for Cancer Research    http://www.aacr.org/ 
American Chemical Society    http://www.chemistry.org/portal/a/c/s/1/home.html 
American Society for Mass Spectrometry    http://www.asms.org/ 
 



Research/Clinical Interest
Title
Director, UAB CCC Mass Spectrometry/ Proteomics Shared Facility
Description
Dr. Mobley is presently employing various peptide and PTM-selective quantitative methods, which include the use of non-tag and stable isotope tags such as is found in commercial kits for SILAC, AQUA, and iTRAQ to select for and map changes in peptidomic and post translational events such as phosphorylations. As a recent recruit to UAB Dr. Mobley was awarded funds from various avenues to develope a Urologic research center with a focus on Clinical Proteomic driven biomarker discovery using the latest tools in Mass Spectrometry.

Selected Publications 
Publication PUBMEDID
Norris J.L., Cornett S., Mobley J.A., Seeley E.H., Roder H., Chaurand P., Caprioli R.M. Preparing MALDI TOF Mass Spectra for Statistical Analysis: A Practical Approach (IJMS, 260, 2-3, 1Feb 2007, pp. 221-221).   17541451 
Islam M.M., Wallin R.. Wynn R.M., Conway M., Fujii H., Mobley J.A., Chuang D.T., Hutson S.M., A Novel Branched-Chain Amino Acid Metabolon: Protein-Protein Interactions in a Supramolecular Complex, JBC, 2007, Feb 21.  17314104 
Chaurand P., Norris J.L., Cornett D.S., Mobley J.A., Caprioli R.M., New Developments in Profiling and Imaging of Proteins from Tissue Sections by MALDI Mass Spectrometry, J Proteome Res. 2006 Nov;5(11):2889-900.  17081040 
Amann J.M., Chaurand P., Gonzalez A., Mobley J.A., Massion P. Caprioli1 R.M., Carbone D.P. Selective Profiling of Proteins in Cancer Cells from Fine Needle Aspirates by MALDI-TOF Mass Spectrometry, Clin Cancer Res. 2006 Sep 1;12(17):5142-50.  16951232 
Cornett D.S., Mobley J.A., Dias, E.C., Andersson M., Arteaga C.L., Sanders M.E., Caprioli, R.M.; Histology Directed MALDI-MS Profiling Improves Throughput and Cellular Specificity in Human Breast Cancer, Mol Cell Proteomics. 2006 Oct;5(10):1975-83. Epub 2006 Jul 18.  16849436 
Lam YW, Mobley JA, Ho SM. Identification of the Antiangiogenic Cytokine, Platelet Factor 4 as a Marker for Metastatic Prostate Cancer Through Protein Profiling Combined with Multidimensional Separation Of The Serologic Proteome. Proteomics. 2005 Jul;5(11):2927-38  15952230 
Yet-Kin Lau, Mobley JA, Lau KM, Ho SM. A Cytochrome P450 1A1 Variant is Over-Expressed in Ovarian Cancer Cell lines and Tissues, Cancer Res. 2005 May 1;65(9):3726-34.  15867368  
Zielie PJ, Mobley JA*, Ebb R, Jiang Z, Ho SM. A Novel Diagnostic Test for Prostate Cancer Using RT-PCR to Determine α-Methylacyl-CoA Racemase Levels in Prostatic Secretions. Journal of Urology, Sept 2004 (* - Equal contribution to first Author)  15311056 
Mobley JA, Lam YW, Lau KM, Pais VM, L’Esperance JO, Steadman B, Burgos Fuster LM, Blute RD, Zielie PJ, Taplin ME, Ho SM. Diagnosing Prostate Cancer Through Changes in the Low Molecular Weight Serologic Proteome. Journal of Urology, July 2004.  15201806 
Mobley JA, L’Esperance JO, Wu M, Hanson RH, Ho SM. A Novel Estrogen 17-20Z-21-[(4-Amino)Phenyl]-19-Norpregna-1,3,5(10),20-Tetraene-3,17-Diol Induces Apoptosis in Prostate Cancer Cell Lines at Nanomolar Concentrations In Vitro, Molecular Cancer Therapeutics, 2004, May;3(5).  15141016 
Mobley JA, Brueggemeier RW, Estrogen Receptor Mediated Increased Sensitivity to DNA Damage In Breast Cancer Cell Lines. Carcinogenesis. 2004 Jan;25(1):3-9. Epub 2003 Sep 26.  14514655 
Mobley JA, Leav I, Wotkowitz C, Lam Y, Zielie P, Evans J, L’Esperance B, Jiang Z, Ho SM, Branched Chain Fatty Acids in Dairy and Beef Products Enhance -MethylAcyl-CoA-Racemace in Prostate Cancer Cells In-Vitro. Cancer Epidemiol Biomarkers Prev. 2003 Aug;12(8):775-83  12917210 
Kim YC, Mobley JA, Brueggemeier RW, Synthesis and Estrogen Receptor Binding Affinities of 7-Hydroxy-3-(4-hydroxyphenyl)-4H-1-Benzopyran-4-Ones Containing a Basic Side Chain, Bioorganic Medicinal Chemistry Letters, 2003; 13 (8).  12668015  
Brueggemeier RW, Gu X, Mobley JA, Joomprabutra S, Bhat AS, Whetstone JL, Effects of phytoestrogens and synthetic combinatorial libraries on aromatase, estrogen biosynthesis, and metabolism. Ann N Y Acad Sci. 2001 Dec; 948:51-66.  11795395  
Mobley JA, Brueggemeier RW, Increasing the DNA Damage Threshold in a Breast Cancer Cell Line. Tox. & Appl. Pharm. 2002; 180 (3) 219-226.   10077490 
Mobley JA, Brueggemeier RW, Measurement of Oxidative DNA Damage by Catechol Estrogens and Analogs In-Vitro. Chem. Research.& Tox. 1999; 12(3); 270-277.  10077490 
Chaurand, P., Rahman M.A., Hunt T., Mobley J.A., Gu G., Caprioli R.M. and Kasper S. Monitoring Mouse Prostate Development by Profiling and Imaging Mass Spectrometry. Mol Cell Proteomics. 2007 (Submitted)   
Chang J.R., Poliakov A., Prevelige P.E., Mobley J.A. and Dokland T., Incorporation of scaffolding protein gpO in Bacteriophages P2 and P4, J. Virology, 2007 (accepted)   
Caldwell RL, Holt GE, Bradbury T, Mobley JA, Zimmerman LJ, Schwartz HS, and Caprioli RM. Proteomic Profiling by MALDI Mass Spectrometry Distinguishes Clinical Grades of Soft Tissue Sarcomas (Re-submitted to Molecular and Cellular Proteomics)   
Pais V, Dahl D, Trainer A, Mobley J, Gallagher K, Demetrius L, Blute R. Evaluation of Surgical Margins Achieved by Laparoscopic Radical Prostatecomy Am. Col. Surg. 2001 Supp. Vol. LII: 600.   
 

Keywords
Cancer, Prostate, Pancreatic, Breast, Biomarkers, Mass Spectrometry