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Faculty Detail    
Campus Address MCLM 395A Zip 0005
Phone  205-934-1002
Other websites

Graduate  Medical University of South Carolina    1986  PhD 

Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Cell, Developmntl, & Integrative Biology  Cell, Developmntl, & Integrative Biology Professor
Secondary  Microbiology  Microbiology Professor
Center  Center for Biophysical Sciences/Engineering  Center for Biophysical Sciences/Engineering Professor
Center  Comp Arthritis, MSK, Bone & Autoimmunity Ctr  Comp Arthritis, MSK, Bone & Autoimmunity Ctr Professor
Center  Comprehensive Cancer Center  Comprehensive Cancer Center Professor
Center  Cystic Fibrosis Research Center  Cystic Fibrosis Research Center Professor
Center  GL Ctr for Craniofacial, Oral, & Dental Disorders  GL Ctr for Craniofacial, Oral, & Dental Disorders Professor
Center  General Clinical Research Center  Nephrology Research & Training Center Professor

Graduate Biomedical Sciences Affiliations
Cell, Molecular, & Developmental Biology 
Cellular and Molecular Biology Program 
Medical Scientist Training Program 
Pathobiology and Molecular Medicine 

Biographical Sketch 
James F. Collawn, Professor of Cell Biology, received his B.S. degree from the University of South Carolina and his Ph.D. degree in Biochemistry from the Medical University of South Carolina (1986). He completed his postdoctoral training in cellular immunology at The Scripps Research Institute and in cell biology at The Salk Institute in La Jolla, California. After working for two years as a Staff Scientist at The Salk Institute, Dr. Collawn joined the Department of Cell Biology in 1993. Dr. Collawn became an Established Investigator of the American Heart Association and Director of the Cell Biology Graduate Program in 2000.

Society Memberships
Organization Name Position Held Org Link
American Physiological Society     

Research/Clinical Interest
Role of ion channels in cystic fibrosis
Our laboratory studies receptor-mediated endocytosis (RME) and protein trafficking. Our studies examine the polarized trafficking of two integral membrane proteins in epithelial cells, the transferrin receptor (TR) and the cystic fibrosis transmembrane conductance regulator (CFTR). TR is expressed in nearly all mammalian cells and its itinerary is well characterized by us and others. CFTR is a cAMP activated chloride channel and its transit through the secretory and endocytic pathways is just beginning to be understood. Cystic fibrosis (CF), the most common genetic disorder in the Caucasian population, results from defective processing or function of the CFTR protein. Therapeutic approaches have focused on increasing the amount or improving the function of defective CFTR at the apical membrane. However, until the exact physiological functions and trafficking pathways of wild-type CFTR have been characterized, such treatments remain only empirical. Using biochemical and physiological approaches, we are studying the cellular mechanisms that regulate wild-type CFTR biogenesis, endocytosis, recycling and function. We are also comparing the above features of the wild type protein to naturally-occurring CFTR mutants in order to understand the metabolic and functional defects in these proteins that result in a certain disease phenotype.

Selected Publications 
Publication PUBMEDID
Collawn, J. F., and Matalon, S. (2012) The role of CFTR in transepithelial liquid transport in pig alveolar epithelia. Am J Phyiol Lung Cell Mol Physiol. 303:L489-91.  22797251 
Collawn, J. F., and Matalon, S. (2012) The role of CFTR in transepithelial liquid transport in pig alveolar epithelia. Am J Physiol Lung Cell Mol Physiol. 303:L489-91.  22797251 
Collawn, J. F., Lazrak, A., Bebok, Z., and Matalon, S. (2012) The CFTR and ENaC Debate – How Important is ENaC in CF Lung Disease? Am J Physiol Lung Cell Mol Physiol. 302:L1141-6.   22492740 
Bartoszewski, R., Brewer, J., Rab, A., Crossman, D. K., Bartoszewska, S., Kapoor, N., Fuller, C., Collawn, J. F., and Bebok, Z. (2011) The unfolded protein response (UPR)-activated transcription factor XBP1 induces micro-RNA-346 expression that targets the human antigen peptide transporter 1 (TAP1) mRNA and governs immune regulatory genes. J. Biol. Chem. 286:41862-70.   22002058 
Fu, L., Rab, A., Tang, L. P., Rowe, S. M., Bebok, Z., and Collawn, J. F. (2012) Dab2 is a key regulator of endocytosis and post-endocytic trafficking of the cystic fibrosis transmembrane conductance regulator. Biochem J. 441:633-643.   21995445 
Lazrak, A., Jurkuvenaite, A., Chen, L., Keeling, K. M., Collawn, J. F., Bedwell, D. M., and Matalon, S. (2011) Enhancement of alveolar epithelial sodium channel activity with decreased cystic fibrosis transmembrane conductance regulator expression in mouse lung. Am. J. Physiol Lung Cell Mol Physiol. 301:L557-567.   21743028 
Bai, M., Gad, H., Turacchio, G., Cocucci, E., Yang, J-S., Li, J., Beznoussenko, G. V., Nie, Z., Luo, R., Fu, L., Collawn, J. F., Kirchhausen, T., Luini, A., and Hsu, V. W. (2011) ARFGAP1 promotes AP-2-dependent endocytosis. Nature Cell Biol. 13:559-67.  21499258 
Bartoszewski, R., Rab, A., Fu, L., Bartoszewska, S., Collawn, J. F., and Bebok, Z. (2011) The unfolded protein response and its effect on CFTR expression. Methods in Enzymology 491:3-24.   21329791 
Collawn, J. F., Fu, L., and Bebok, Z. (2010) Targets for cystic fibrosis therapy: proteomic analysis and correction of mutant cystic fibrosis transmembrane conductance regulator. Expert Rev. Proteomics 7:495-506.   20653506 
Bartoszewski, R. A., Jablonsky, M., Bartoszewska, S., Stevenson, L., Dai, Q., Kappes, J., Collawn, J. F., and Bebok, Z. (2010) A synonymous single nucleotide polymorphism in DeltaF508 CFTR enhances the expression of the mutant protein. J. Biol. Chem. 285:28741-48.   20628052 
Rowe, S. M., Pyle, L. C., Jurkuvenaite, A., Varga, K., Collawn, J. F., Sloane, P. A., Woodworth, B., Mazur, M., Fan, L., Yao, L., Fortenberry, J., Sorscher, E. J., and Clancy, J. P. (2010). DeltaF508 CFTR Processing Correction and Activity in Polarized Airway and Non-Airway Cell Monolayers. Pulm. Pharm. and Therapeutics 23:268-78.   20226262 
Jurkuvenaite, A., Chen, L., Bartoszewski, R., Goldstein, R., Bebok, Z., Matalon, S., and Collawn, J. F. (2010) Functional stability of rescued DeltaF508 Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Airway Epithelial Cells. Am J Respir Cell Mol. Biol. 42:363-72.   19502384 

Cystic Fibrosis; Endocytosis; Protein Trafficking, chymase, heart failure