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Faculty Detail    
Name SUBHASHINI BOLISETTY
Associate Professor		  
Campus Address ZRB 614 Zip 0007
Phone  (205) 996-1537
E-mail  sbolisetty@uabmc.edu
Other websites
     

Education
Graduate  University of Alabama at Birmingham    2010  PhD 


Faculty Appointment(s)
Appointment Type Department Division Rank
Center  Nephrology Research & Training Center  Nephrology Research & Training Center Associate Professor
Center  GL Ctr for Craniofacial, Oral, & Dental Disorders  GL Ctr for Craniofacial, Oral, & Dental Disorders Associate Professor
Primary  Med - Nephrology  Med - Nephrology Associate Professor
Secondary  Cell, Developmntl, & Integrative Biology  Cell, Developmntl, & Integrative Biology Instructor

Graduate Biomedical Sciences Affiliations
Biochemistry and Molecular Genetics Program 
Biochemistry and Structural Biology 
Cell, Molecular, & Developmental Biology 

Biographical Sketch 
Dr. Bolisetty received her bachelors in Biotechnology from Anna University in Chennai, India and later received her doctorate degree in Cell Biology under the guidance of Dr. Anupam Agarwal from the University of Alabama at Birmingham. She continued her postdoctoral research at UAB under the guidance of Dr. James George in the Department of Surgery. She then returned to the Division of Nephrology where she is currently an Associate Professor. Dr. Bolisett's research has been continually funded by various organizations, including the NIH, AHA and ASN.

Society Memberships
Organization Name Position Held Org Link
American Heart Association  Communications Committee   
American Physiological Society  Awards Committee   
American Society of Nephrology  Education Committee   
International Society of Nephrology     
 



Research/Clinical Interest
Title
Mechanisms of injury and repair during Acute Kidney Injury
Description
AKI is a common unavoidable consequence, occurring in nearly 2% of all hospitalized patients. Therapy for AKI has remained elusive over the past few decades, underscoring the urgent need for identification of potential therapeutic targets. Following injury, the cascade of events leading to endothelial dysfunction, tubular epithelial injury and activation of tissue-resident and infiltrating macrophages consists of coordinated action of cytokines/chemokines and reactive oxygen species. In this connection, we have previously demonstrated that expression of H-ferritin (FtH) in the kidney is imperative for protection against AKI. FtH is an anti-oxidant that catalyzes the conversion of the potentially toxic ferrous ion to ferric ion and allows for safe incorporation of iron into the ferritin shell and thereby attenuating participation of free iron in ROS generation. My current research efforts are aimed at determining the mechanisms of ferritin mediated cytoprotection and cross-talk between the tubules and resident/infiltrating immune cells during injury. Toward these efforts, we have generated cell-specific ferritin knockout mice to evaluate their effect on injury-mediated inflammation and recovery responses during sepsis and AKI. We elucidate differential regulation of the omics - transcriptome, proteome and metabolome to identify key pathways that are modulated during injury. These studies will provide significant and multi-faceted understanding of the major cellular components (tubular epithelium and macrophages) that are involved in the injury mediated responses following AKI and therefore, provide a potential repertoire of targeted therapeutics for AKI and other immunologically driven diseases.

Selected Publications 
Publication PUBMEDID
Zarjou A, Black LM, McCullough KR, Hull TD, Esman SK, Boddu R, Varambally S, Chandrashekar DS, Feng W, Arosio P, Poli M, Bolisetty S. Ferritin light chain confers protection against sepsis-induced inflammation and organ Injury. Front Immunol. 2019 Feb 4;10:131.   PMC6371952 
Radadiya PS, Thornton MM, Puri RV, Yerrathota S, Dinh-Phan J, Magenheimer B, Subramaniam D, Tran PV, Zhu H, Bolisetty S, Calvet JP, Wallace DP, Sharma M. Ciclopirox olamine induces ferritinophagy and ameliorates disease progression in Polycystic kidney disease. JCI Insight. 2021 Mar 30;6(8):e141299.   PMC8119220 
Black LM, Farrell ER, Barwinska D, Osis G, Zmijewska AA, Traylor AM, Esman SK, Bolisetty S, Whipple G, Kamocka MM, Winfree S, Spangler DR, Khan S, Zarjou A, El-Achkar TM, Agarwal A. VEGFR3 tyrosine kinase inhibition aggravates cisplatin nephrotoxicity. Am J Physiol Renal Physiol. 2021 Dec 1;321(6):F675-F688.   PMC8714977 
Mccullough KR, Akhter J, Taheri MJ, Traylor A, Zmijewska A, Hudson MC, Erman E, Moore K, George JF and Bolisetty S. Functional Consequence of Myeloid Ferritin Heavy Chain on acute and chronic effects of rhabdomyolysis-induced kidney injury. Front Med (Lausanne). 2022 Sep 8;9:894521.   PMC9492979 
Mitochondria and reactive oxygen species: physiology and pathophysiology.
Bolisetty S, Jaimes EA.
Int J Mol Sci. 2013 Mar 19;14(3):6306-44.  		 23528859  
11.
Mitochondria-targeted heme oxygenase-1 decreases oxidative stress in renal epithelial cells.
Bolisetty S, Traylor A, Zarjou A, Johnson MS, Benavides GA, Ricart K, Boddu R, Moore RD, Landar A, Barnes S, Darley-Usmar V, Agarwal A.
Am J Physiol Renal Physiol. 2013 Aug 1;305(3):F255-64. 		 23720344  
Adaptive responses to tissue injury: role of heme oxygenase-1.
Agarwal A, Bolisetty S.
Trans Am Clin Climatol Assoc. 2013;124:111-22.  		 23874015  
Proximal tubule H-ferritin mediates iron trafficking in acute kidney injury.
Zarjou A, Bolisetty S, Joseph R, Traylor A, Apostolov EO, Arosio P, Balla J, Verlander J, Darshan D, Kuhn LC, Agarwal A.
J Clin Invest. 2013 Oct;123(10):4423-34 		 24018561  
Heme Oxygenase-1 Regulates Myeloid Cell Trafficking in AKI.
Hull TD, Kamal AI, Boddu R, Bolisetty S, Guo L, Tisher CC, Rangarajan S, Chen B, Curtis LM, George JF, Agarwal A.
J Am Soc Nephrol. 2015 Sep;26(9):2139-51.  		 25677389  
Heme oxygenase-1 protects corexit 9500A-induced respiratory epithelial injury across species.
Li FJ, Duggal RN, Oliva OM, Karki S, Surolia R, Wang Z, Watson RD, Thannickal VJ, Powell M, Watts S, Kulkarni T, Batra H, Bolisetty S, Agarwal A, Antony VB.
PLoS One. 2015 Apr 2;10(4):e0122275.  		 25835394  
Macrophage and epithelial cell H-ferritin expression regulates renal inflammation.
Bolisetty S, Zarjou A, Hull TD, Traylor AM, Perianayagam A, Joseph R, Kamal AI, Arosio P, Soares MP, Jeney V, Balla J, George JF, Agarwal A.
Kidney Int. 2015 Jul;88(1):95-108. 		 25874599  
Leucine-rich repeat kinase 2 deficiency is protective in rhabdomyolysis-induced kidney injury.
Boddu R, Hull TD, Bolisetty S, Hu X, Moehle MS, Daher JP, Kamal AI, Joseph R, George JF, Agarwal A, Curtis LM, West AB.
Hum Mol Genet. 2015 Jul 15;24(14):4078-93.  		 25904107  
Heme Attenuation Ameliorates Irritant Gas Inhalation-Induced Acute Lung Injury. Aggarwal S, Lam A, Bolisetty S, Carlisle MA, Traylor A, Agarwal A, Matalon S.
Antioxid Redox Signal. 2016 Jan 10;24(2):99-112. 		 26376667 
Proximal tubule-targeted heme oxygenase-1 in cisplatin-induced acute kidney injury. Bolisetty S, Traylor A, Joseph R, Zarjou A, Agarwal A.
Am J Physiol Renal Physiol. 2016 Mar 1;310(5):F385-94. 		 26672618 
 

Keywords
Ferritin, iron, heme oxygenase, macrophages, inflammation, acute kidney injury, sepsis