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Faculty Detail    
Name LUCAS D POZZO-MILLER
Professor
Associate Director, Comprehensive Neuroscience Center
 
Campus Address SHEL 1002 Zip 2182
Phone  205-975-4659
E-mail  lucaspm@uab.edu
Other websites
     


Faculty Appointment(s)
Appointment Type Department Division Rank
Primary  Neurobiology  Neurobiology Professor
Secondary  Cell, Developmntl, & Integrative Biology  Cell, Developmntl, & Integrative Biology Professor
Secondary  Neurology   Neurology Chair Office Professor
Secondary  Psychology  Psychology Professor
Center  Civitan International Research Center  Civitan International Research Center Professor
Center  General Clinical Research Center  Comprehensive Neuroscience Center Professor
Center  Ctr for Glial Bio in Med  Ctr for Glial Bio in Med Professor
Center  Vision Sciences  Vision Science Research Center Professor

Graduate Biomedical Sciences Affiliations
Cell, Molecular, & Developmental Biology 
Cellular and Molecular Biology Program 
Genetics, Genomics and Bioinformatics 
Integrative Biomedical Sciences 
Medical Scientist Training Program 
Neuroscience 
Neuroscience Graduate Program 
Neurosciences 

Biographical Sketch 
Lucas Pozzo-Miller completed his undergraduate studies in Biology (MS, 1986) and earned his graduate degree in Neurobiology from the Universidad Nacional de Córdoba, Argentina (PhD, 1989). He trained as postdoctoral fellow at Case Western Reserve University, Cleveland OH, with Dennis Landis (1990-1992), and at the Roche Institute of Molecular Biology, Nutley NJ, with John Connor (1992-1995). He performed research at the Marine Biological Laboratory, Woods Hole MA, with National Academy member Rodolfo Llinás (1994 Grass Foundation Fellow, and 1995 Lakian Foundation Fellow). He held a Senior Staff Fellow position (research assistant professor) at the National Institutes of Health, Bethesda MD, in the laboratory of National Academy member Tom Reese (1995-1998). Dr. Pozzo-Miller joined the faculty of the Department of Neurobiology at the University of Alabama at Birmingham in 1998. Currently, he is Professor of Neurobiology and Associate Director of the Comprehensive Neuroscience Center.

Society Memberships
Organization Name Position Held Org Link
Society for Neuroscience  Member  http://web.sfn.org/ 
The American Physiological Society  Member  http://www.the-aps.org/index.htm 

Research/Clinical Interest
Title
Neurotrophins in Synapse Development and Plasticity, and Intellectual Disabilities (e.g. Rett Syndrome)
Description
Our long-term interest is to characterize the functional role of different cellular compartments in neurons, such as dendritic spines and presynaptic terminals, and how they participate in the development, function, and plasticity of synapses as they relate to learning & memory and social behaviors. Neurotrophins, like brain-derived neurotrophic factor (BDNF), are secretory proteins that regulate the survival and differentiation of neurons during early brain development, and also synapse function and plasticity throughout the lifetime. We investigate the “BDNF hypothesis” of Rett syndrome, a neurodevelopmental disorder associated with autistic features and intellectual disabilities that is caused by mutations in MECP2, a transcriptional regulator of many genes, including Bdnf. We use transgenic mice that express mutant Mecp2 as experimental models to characterize the molecular and cellular pathophysiology of Rett syndrome, and to test novel therapeutic approaches. In our studies, we apply several experimental approaches, including intracellular whole-cell recordings, voltage-sensitive dye and intracellular Ca2+ imaging, confocal microscopy, time-lapse imaging, multiphoton excitation microscopy, electron microscopy, and machine-learning behavioral assays.

Positions Available
Date Posted Position Title
4/12/2016  Research Assistant (lab technician) 
Full-time lab technician to perform:

1. Management of transgenic mouse colony (genotyping by PCR, breeding, record keeping).
2. Cell and tissue culture of primary neurons and brain slices.
3. Western blots and RT-qPCR.
4. General lab solutions (e.g. buffers, culture media).

We will provide training in all those techniques. Must have at least hands-on experience with mice.

Available immediately.
   

Selected Publications 
Publication PUBMEDID
Li W, Xu X, Pozzo-Miller L. Excitatory synapses are stronger in the hippocampus of Rett syndrome mice due to altered synaptic trafficking of AMPA-type glutamate receptors. Proc Natl Acad Sci U S A. 2016 Feb 29. pii: 201517244. [Epub ahead of print]  26929363 
Pozzo-Miller L, Pati S, Percy AK. Rett Syndrome: Reaching for Clinical Trials. Neurotherapeutics. 2015 Jul;12(3):631-40.  25861995 
Phillips M, Pozzo-Miller L. Dendritic spine dysgenesis in autism related disorders. Neurosci Lett. 2015 Aug 5;601:30-40.  25767435 
Xu X, Miller EC, Pozzo-Miller L. Dendritic spine dysgenesis in Rett syndrome. Front Neuroanat. 2014 Sep 10;8:97.  25309341 
Calfa G, Li W, Rutherford JM, Pozzo-Miller L. Excitation/inhibition imbalance and impaired synaptic inhibition in hippocampal area CA3 of Mecp2 knockout mice. Hippocampus. 2015 Feb;25(2):159-68.  25209930 
Xu X, Kozikowski AP, Pozzo-Miller L. A selective histone deacetylase-6 inhibitor improves BDNF trafficking in hippocampal neurons from Mecp2 knockout mice: implications for Rett syndrome. Front Cell Neurosci. 2014 Mar 7;8:68.  24639629 
Li W, Pozzo-Miller L. BDNF deregulation in Rett syndrome. Neuropharmacology. 2014 Jan;76 Pt C:737-46.  23597512 
Li W, Calfa G, Larimore J, Pozzo-Miller L. Activity-dependent BDNF release and TRPC signaling is impaired in hippocampal neurons of Mecp2 mutant mice. Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):17087-92.   23027959 
Chapleau CA, Boggio EM, Calfa G, Percy AK, Giustetto M, Pozzo-Miller L. Hippocampal CA1 pyramidal neurons of Mecp2 mutant mice show a dendritic spine phenotype only in the presymptomatic stage. Neural Plast. 2012;2012:976164.   22919518 
Leuner K, Li W, Amaral MD, Rudolph S, Calfa G, Schuwald AM, Harteneck C, Inoue T, Pozzo-Miller L. Hyperforin modulates dendritic spine morphology in hippocampal pyramidal neurons by activating Ca(2+) -permeable TRPC6 channels. Hippocampus. 2013 Jan;23(1):40-52.  22815087 
Amaral MD, Pozzo-Miller L. Intracellular Ca2+ stores and Ca2+ influx are both required for BDNF to rapidly increase quantal vesicular transmitter release. Neural Plast. 2012;2012:203536.  22811938 
Hartmann D, Drummond J, Handberg E, Ewell S, Pozzo-Miller L. Multiple approaches to investigate the transport and activity-dependent release of BDNF and their application in neurogenetic disorders. Neural Plast. 2012;2012:203734.   22720171 
Chapleau CA, Pozzo-Miller L. Divergent roles of p75NTR and Trk receptors in BDNF's effects on dendritic spine density and morphology. Neural Plast. 2012;2012:578057.  22548193 
Li W, Pozzo-Miller L. Beyond Widespread Mecp2 Deletions to Model Rett Syndrome: Conditional Spatio-Temporal Knockout, Single-Point Mutations and Transgenic Rescue Mice. Autism Open Access. 2012;2012(Suppl 1):5  23946910 
Calfa G, Chapleau CA, Campbell S, Inoue T, Morse SJ, Lubin FD, Pozzo-Miller L. HDAC activity is required for BDNF to increase quantal neurotransmitter release and dendritic spine density in CA1 pyramidal neurons. Hippocampus. 2012 Jul;22(7):1493-500.  22161912 
Calfa G, Hablitz JJ, Pozzo-Miller L. Network hyperexcitability in hippocampal slices from Mecp2 mutant mice revealed by voltage-sensitive dye imaging. J Neurophysiol. 2011 Apr;105(4):1768-84.  21307327 
Calfa G, Percy AK, Pozzo-Miller L. Experimental models of Rett syndrome based on Mecp2 dysfunction. Exp Biol Med (Maywood). 2011 Jan;236(1):3-19.  21239731 
Li Y, Calfa G, Inoue T, Amaral MD, Pozzo-Miller L. Activity-dependent release of endogenous BDNF from mossy fibers evokes a TRPC3 current and Ca2+ elevations in CA3 pyramidal neurons. J Neurophysiol. 2010 May;103(5):2846-56.  20220070 
Chapleau CA, Calfa GD, Lane MC, Albertson AJ, Larimore JL, Kudo S, Armstrong DL, Percy AK, Pozzo-Miller L. Dendritic spine pathologies in hippocampal pyramidal neurons from Rett syndrome brain and after expression of Rett-associated MECP2 mutations. Neurobiol Dis. 2009 Aug;35(2):219-33.  19442733 
Larimore JL, Chapleau CA, Kudo S, Theibert A, Percy AK, Pozzo-Miller L. Bdnf overexpression in hippocampal neurons prevents dendritic atrophy caused by Rett-associated MECP2 mutations. Neurobiol Dis. 2009 May;34(2):199-211.  19217433 

Keywords
BDNF, Ca2+, Synapse, Rett Syndrome, Hippocampus, Dendritic Spines, MeCP2, Autism, Intellectual Disability