UASOM Faculty Profiles


Name	MATTHEW B. RENFROW
Campus Address	KAUL 524 Zip 0024
Phone	(205) 996-4681
E-mail	renfrow@uab.edu
Other websites	Research Lab website Biomedical FT-ICR Lab BSB theme profile

Undergraduate

Western Kentucky University

1996

B.S. Biochemistry & Recombinant Genetics

Graduate

University of Georgia

2002

Ph.D. Biochemistry & Molecular Biology

Fellowship

National High Magnetic Field Laboratory, Florida State University

2004

Postdoctoral research, Fourier transform-mass spectrometry

Appointment Type

Department

Division

Rank

Primary

Biochemistry & Molecular Genetics

Professor

Center

Center for AIDS Research

Professor

Center

Comprehensive Cancer Center

Professor

Center

Med - Cardiovascular Disease

Ctr Cardiovasc Bio (Org Ret)

Professor

Center

Nephrology Research & Training Center

Professor

Biochemistry and Molecular Genetics Program

Biochemistry and Structural Biology

Cancer Biology

Microbiology

Matt was born and raised in Bowling Green, KY. He obtained his B.S. in biochemistry and recombinant genetics from Western Kentucky University in 1996. As a Hilltopper undergraduate he worked in his dad’s pharmacy as a pharmacy technician and as a technician in the WKU Coal and Fuel Laboratory performing heavy metal analysis on coal by use of an ICP-AES spectrometer. As a graduate student at the University of Georgia (1996-2002), he studied transcription initiation in the hyperthermophile, Pyrococcus furiosus, performing protein-DNA crosslinking experiments on the transcription pre-initiation complex. For his postdoctoral studies at the National High Magnetic Field Laboratory at Florida State University (2002-2004), Matt returned to his analytical roots and studied Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry on proteins and peptides with emphasis on ion fragmentation techniques that allow for characterization of post-translational modifications such as glycosylation and phosphorylation. Since arriving at UAB in 2004, he has continued to develop methodologies for the analysis of modified proteins and peptides by high resolution mass spectrometry with emphasis on defining protein glycosylation heterogeneity in disease related proteins.

Organization Name

Position Held

Org Link

American Society for Mass Spectrometry

member

http://www.asms.org/

American Society for Nephrology

member

http://www.asn-online.org/

Title
Analysis of Protein Glycosylation in Human Disease by High Resolution Mass Spectrometry
Description
Dr. Renfrow's research interests are focused around the use of advanced mass spectrometry techniques for structural analysis of biomolecules. Specifically, the use of high resolution Fourier transform-mass spectrometry (FT-MS) in the analysis of protein glycosylation heterogeneity, especially the clustered sites of O-glycosylation found in IgA1. Patients with IgA nephropathy (IgAN), a chronic kidney disease that often leads to end stage renal disease (ESRD), have altered IgA1 O-glycans that are involved in the pathogenesis of the disease and could serve as biomarkers for the diagnosis and possibly prognosis of the disease. Dr. Renfrow's laboratory has developed several novel approaches for the systematic analysis of IgA1 O-glycosylation heterogeneity to identify the various sites of attachment and which sites show the aberrancy relative to healthy controls. The techniques developed have been applied to a wide range of protein glycosylation including the changes in N-glycans found in the HIV env protein gp120 as well as a variety of other glycosylated proteins.

Publication	PUBMEDID
Novak, J., Renfrow, M.B., Gharavi, A.G., Julian, B.A. “Pathogenesis of immunoglobulin A nephropathy” (2013) Curr Opin Nephrol Hypertens. Mar 18.epub.	23511518
Takahashi, K., Smith, A.D., IV, Julian, B.A., Mestechy, J., Novak, J., Renfrow, M.B., “Identification of Native Structural Isomers in IgA1 Hinge-region O-Glycosylation Using High-resolution Mass Spectrometry” (2012) J. Prot. Res., Feb 3;11:692-702.	22067045
Suzuki, H., Kiryluk, K., Novak, J., Moldoveanu, Z., Herr, A.B., Renfrow M. B., Wyatt, R.J., Scolari, F., Mestecky, J., Gharavi, A.G., Julian, B.A. “The Pathophysiology of IgA Nephropathy” (2011), J. Amer. Soc. Neph., Oct.;22:1795-803.	21949093
Gang, X, Boerma, L.J., Cox, B.D., Qiu, C., Kang, S., Smith, C.D., Renfrow, M.B.#, Muccio, D.D.#,, “Structure, Energetics, and Dynamics of Binding Coactivator Peptide to Human Retinoid X Receptor Alpha Ligand Binding Domain Complex with 9-cis-Retinoic Acid” (2011) Biochemistry,50(1);93-105.	21049972
Takahashi, K., Wall, S.B., Suzuki, H., Hall, S. Smith, A.D.,IV, Poulsen, K., Kilian, M., Julian, B.A., Mestecky, J., Novak, J., Renfrow, M.B. “Clustered O-glycans of IgA1: Defining macro- and micro-heterogeneity by use of electron capture/transfer dissociation.” (2010) Mol. Cell. Proteomics. 9:2545-57.	20823119
Raska, M., Takahashi, K., Hall, S., Moldoveanu, Z., Elliot, Wilson, L., Brown, R., Barnes, S., Tomana, M., Smith, Mestecky, J., Renfrow, M.B., Novak, J. “Glycosylation pattern of HIV-1 gp120 is cell specific and affects binding of neutralizing antibodies. (2010) J. Biol. Chem., 285:20860-9.	20439465
Renfrow, M.B., MacKay, C.L., Chalmers, M.J., Julian, B.A., Mestecky, J., Kilian, M., Poulsen, K., Emmett, M.R., Marshall, A.G., Novak, J. “Analysis of O-glycan Heterogeneity in IgA1 Myeloma Proteins by Fourier Transform Ion Cyclotron Resonance Mass Spectrometry: Implications for IgA Nephropathy.” (2007) Analytical and Bioanalytical Chemistry, 389:1397-407.	17712550
Renfrow, M.B., Cooper, H.J., Kulhavy, R., Tomana, M., Emmett, M.R., Mestecky, J., Marshall, A.G., Novak, J. “Determination of aberrant O-glycosylation in the IgA1 hinge region by electron capture dissociation Fourier transform-ion cyclotron resonance mass spectrometry” (2005) J. Biol. Chem., 280:19136-45.	15728186

Fourier transform mass spectrometry (FT-MS), protein glycosylation, IgA O-glycosylation, IgA nephropathy, proteomics, glycoproteomics